A new RP-HPLC method for lamivudine has been developed and validated. The developed method was simple and accurate and validated for various parameters as per ICH guidelines. The drug has shown �»max at 226 nm in UV. The linearity concentration range for the drug was found to be 100-500 �¼g/ml and co-relation coefficient was found to 0.998 for RP-HPLC method respectively. The validation parameters as per the ICH guidelines were determined and found to be within the limits.
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